Coding

Part:BBa_K1331004

Designed by: Tianyi Huang   Group: iGEM14_Nankai   (2014-09-25)
Revision as of 11:46, 17 October 2014 by Danielle D (Talk | contribs)

Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6

This part encodes rhamnosyltransferase I subunit B.

It includes the whole coding sequence,a part of upstream noncoding sequence and a part of downstreanm noncoding sequence of rhlB.

The coding sequence of our part is similar with Part:BBa_K317999[1] designed by iGEM10_Tokyo-NoKoGen, but two mutations have been done to remove the PstI restriction sites(at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.


Usage and Biology

RhlB together with rhlA[2] encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 758
    Illegal NgoMIV site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
None