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Part:BBa_K1463000

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-09-09)
Revision as of 12:08, 13 October 2014 by Cakesjason (Talk | contribs)

Recombinase Switch With GFP and RFP

A recombinase switch with GFP one side of the switch and RFP on the other.

Fig. 1: BL21 E. coli carrying different constructs on [http://2012.igem.org/Team:Goettingen/Project/Materials#M9_Swimming_Agar M9 minimal medium swarming agar]. after 48h incubation at 33°C. Cells expressing fliC in puc18 under the natural promoter travelled approximately 1,5cm (radius) whereas no swimming could be detected for the control plasmid carrying K777125. On tryptone swimming agar yhjH transformants were usually faster.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 813
    Illegal NheI site found at 836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 711
    Illegal BsaI.rc site found at 1668


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