Part:BBa_F2620

Input: 3OC6HSL Output: PoPS

tetR R0040 LuxR I0462 lux pR R0062

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Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Performance

Experiment*	Characteristic*	Value*
Transfer Function	Input Swing	1E-9 - 1E-6 M 3OC6HSL, exogenous
	Output Swing	0±1 - 503±1 GFP molecules cfu-1 s-1
	Switch Point	7E-9 ±1E-9 M 3OC6HSL, exogenous
Response time:	Time from 0 - 50% output	9 min
	Time from 0 - 90% output	27 min
	Time from 100 - 50% output	# min
	Time from 100 - 5% output	# min
Specificity	Strong response to	3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL
	Weak response to	C4HSL, C10HSL, C12HSL
Stability	Genetic Stability (Low/High Input)	>92/74 generations
	Performance Stability (Low/High Input)	>92/74 generations
Demand	Translation Demand: (Low/High Input)	5040/141600 charged tRNA cfu-1 s-1
		336/9440 ribosomes sequestered cfu-1
	Transcription Demand: (Low/High Input)	(#/#) nucleotides cfu-1 s-1
		(#/#) RNAP sequestered cfu-1
	Growth Rate (Low/High Input)	54/59 min doubling time

• Measured by Ania Labno and Barry Canton 2006-2007

Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC₆HSL, C6HSL, C7HSL, C8HSL, C10HSL.