Composite

Part:BBa_K1119011:Design

Designed by: Wai In Hui   Group: iGEM13_Hong_Kong_HKUST   (2013-10-24)
Revision as of 02:58, 29 October 2013 by Wihui (Talk | contribs) (→‎Source)


Human Elongation Factor-1alpha Promoter - GFP - hGH polyA tail


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal AgeI site found at 83
    Illegal AgeI site found at 1067
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1470
    Illegal BsaI.rc site found at 972


Design Notes

There are NgoMIV & AgeI sites around GFP reporter (BBa_K648013), which make the part incompatible with RFC25 standard as shown by Part Registry, but users can perform in-frame protein fusion using the mentioned sites.

Source

HKUST iGEM team 2013

References