Part:BBa_K1031912

J23105-XylS-Terminator

For detailed information concerning XylS, please visit 2013 Peking iGEM Biosensor XylS

Characterization

In order to fine-tune the performance of XylS biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031912 is composed of three elements, the constitutive Pc promoter J23105, coding sequence of XylS and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit Pm/J23105-XylS. Orange arrowheads represent promoters. RBSs are shown as green ovals and squares in dark red stand for B0015 terminator.

[http://2019.igem.org/Team:BHSF_ND BHSF_ND 2019] Characterised the efficiency on the function of translating fluorescence under different inducer concentration at the given time of 240 minutes and under different time stage at a given inducer concentration.Also, we find out that at a certain time node that the system will suddenly increase its productivity. At last, the result suggested that both system have noticeable leakage and Xyls has much higher leakage. Check results on Experience.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 93
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 815
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 278

Data shown

Induction ratio of XylS biosensor library from ON/OFF test that utilizes different Pc promoters to control the expression of XylS. The dashed box refers to data for Pm/J23105-XylS biosensor circuit (Fig 2)

Fig 2 Horizontal axis stands for different XylS biosensor with Pc promoters of different strength. The expression strength of these constitutive promoters, J23113, J23109, J23114, J23105, J23106 is 21, 106, 256, 623, and 1185, respectively, according to the Parts' registry. Four kinds of aromatics, namely BzO, 2-MxeBzO, 3-MeBzO and 4-MeBzO, shown with different color intensities, were tested following Test Protocol 1. Vertical axis represents the ON/OFF induction ratio. The dashed box refers to performance for Pm/J23105-XylS biosensor circuit.