An Introduction to BioBricks
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BioBricks are a set of interchangable parts, designed with a view to building biological systems in living cells. The picture to the left shows BioBrick part BBa_0034 within a plasmid. The yellow arrow represents the plasmid's antibiotic resistance gene and the green arrow, its origin of replication. For more information see:
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Building BioBrick Systems
Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on restriction enzymes, purification, ligation, and transformation - with a twist: BioBrick parts are composable. The result of assembling two parts is a new part that may be used in future assemblies.
- Standard Assembly
- The classical method of BioBrick assembly, done at the lab bench.
- Insert pic of std assembly
- Automated Assembly
- Automated assembly, currently in late stage development at iGEM labs.
- Insert pic of auto assembly
How do I make a BioBrick?
At the bench
Pic of biobrick flanked by coding seq
Methods:
- Synthesis
- PCR
More information on putting sequence into standardized Biobrick form can be found here
Adding the part to the registry
This protocol is quick and easy. A comprehensive guide can be found here