Inverter
pCaiF-TetR

Part:BBa_K1060010

Designed by: Ingmar Claes, Lukas Marcelis, Su Wang   Group: iGEM13_KU_Leuven   (2013-09-19)
Revision as of 09:07, 30 September 2013 by SanderW (Talk | contribs)

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TetR expression under glucose limiting conditions

This device, which is in principle an invertor, produces the tetracyline repressor (TetR) (BBa_C0040) under glucose limiting conditions using the pCaiF promoter (BBa_K398326). The TetR used has an extra LVA tag, resulting in faster degredation and so quicker responses to changes in glucose concentrations. This device was used as an invertor to shut off methyl salicylate production (BBa_K1060003) in the absence of honeydew produced by aphids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None