Device

Part:BBa_K1065309:Design

Designed by: Fabio Digiacomo   Group: iGEM13_UNITN-Trento   (2013-09-18)
Revision as of 13:09, 26 September 2013 by Fabius924 (Talk | contribs) (→‎Design Notes)


light regulated circuit producing amilGFP+EFE at dark


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 842
    Illegal NgoMIV site found at 932
    Illegal NgoMIV site found at 950
    Illegal NgoMIV site found at 1462
    Illegal NgoMIV site found at 1755
    Illegal NgoMIV site found at 1849
    Illegal AgeI site found at 484
    Illegal AgeI site found at 1630
    Illegal AgeI site found at 4060
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1519
    Illegal BsaI.rc site found at 383


Design Notes

We designed this part by inserting the part BBa_K1065302 (using KAPA polymerase for PCR reaction) upstream of EFE coding sequence in BBa_K1065002. so we built a part consisting of the blue light circuit that regulates amilCP+EFE production. Note that a terminator is missing after PfixK2. We suggest to instert at least one terminator after the inducible promoter in order to have a better defined switch from the ON state to the OFF state.

Source

the part sources are: Bba_K1065302 (our improved part) and BBa_K1065002 (EFE+terminators), both submitted in the registry by our team.

References