Translational_Unit

Part:BBa_K1159214

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM13_TU-Munich   (2013-09-17)
Revision as of 16:21, 17 September 2013 by Earthnut (Talk | contribs)

Membrane-anchored SpyTag with secretory nLuc_SpyCatcher reporter fusion translational unit

This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyTag and a secretory nLuc_SpyCatcher (IgKappa-SigP_nLuc_SpyCatcher) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized catcher protein and the secretory fusion protein containing N-terminal nLuc and C-terminal SpyCatcher Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1384
    Illegal XhoI site found at 1880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1148
    Illegal AgeI site found at 1503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 159
    Illegal BsaI.rc site found at 1077
    Illegal SapI.rc site found at 1581


[edit]
Categories
Parameters
None