Coding

Part:BBa_K1060001:Design

Designed by: Lukas Marcelis, Su Wang   Group: iGEM13_KU_Leuven   (2013-09-17)
Revision as of 09:25, 17 September 2013 by IngmarClaes (Talk | contribs) (→‎References)

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EBF synthase from Streptomyces


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 360
    Illegal AgeI site found at 1154
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no EcoRI, XbaI, NotI, SpeI or PstI sites present


Source

Streptomyces coelicolor 3A(2) genomic DNA

References

Bin Zhao, Li Lei, Dmitry G. Vassylyev, Xin Lin, David E. Cane, Steven L. Kelly, Hang Yuan, David C. Lamb, Michael R. Waterman. Crystal Structure of Albaflavenone Monooxygenase Containing a Moonlighting Terpene Synthase Active Site. J Biol Chem. 2009 December 25; 284(52): 36711–36719. Published online 2009 October 26. doi: 10.1074/jbc.M109.064683