RNA

Part:BBa_K1124010:Design

Designed by: Yuta Otsuka   Group: iGEM13_UT-Tokyo   (2013-09-11)
Revision as of 20:18, 27 September 2013 by Minakuchi.T (Talk | contribs)

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anti-csrA sRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was generated by site-directed mutagenesis on MicC-sRNA scaffold(BBa_K1124005), in which the target-binding sequence was added to the scaffold. The target binding sequence was designed complementary to the csrA gene of E. coli K-12 strain.


Source

We designed oligo DNA complementary to the first 24nt of csrA CDS as the target binding sequence. Then, we integrated it with MicC sRNA scaffold (BBa_K1124005) using PCR technique.

References

Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013). Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature biotechnology.

Revelles, O., Millard, P., Nougayrède, J. P., Dobrindt, U., Oswald, E., Létisse, F., & Portais, J. C. (2013). The Carbon Storage Regulator (Csr) System Exerts a Nutrient-Specific Control over Central Metabolism in Escherichia coli Strain Nissle 1917. PloS one, 8(6), e66386.