Measurement

Part:BBa_K880002

Designed by: Josh Atkinson, Mike Ferguson, and Ben Parker   Group: iGEM12_Michigan   (2012-09-29)
Revision as of 14:03, 3 October 2012 by Jatk (Talk | contribs) (→‎A Reporter for Assaying fim-Based Recombinase Activity)

A Fluorescent Reporter for Assaying fim-Based Recombinase Activity

-IRR K137008 + inverted tetR promoter K137047 + IRL K137010 + GFP I13504 (MI275) -GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases.

This has the same components as K137058 with the inverted repeats reversed such that the external half sites are external to the flip region (IRR - “flip region” - IRL)

It is a GFP fluorescent reporter capable of assaying the functionality of fim regulatory recombinases; based on K137058.

K137058’s invertible repeat sequences responsible for recombinase binding are ordered incorrectly; the regions are not identical, and have “left” and “right” components. K137008 (invertible repeat-right) and K137010 (invertible repeat-left) are mislabeled and belong in the order “K137008-region to invert-K137010” in accordance with the wild-type fim system.

Repeat sequence in this part exist in the correct (wild-type) orientation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1006
[edit]
Categories
Parameters
None