Part:BBa_K845008:Design

cyaA with its natural RBS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 288
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 922
  Illegal SapI.rc site found at 412
  Illegal SapI.rc site found at 652
  

Design Notes

This gene has to be inserted in ΔcyaA bacteria.

Safety note: https://static.igem.org/mediawiki/2012/4/48/CyaA.pdf

Source

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References

[1] Gorke, B., & Stulke, J. 2008. Carbon catabolite repression in bacteria: many ways to make the most out of nutrients. Nat Rev Microbiol, 6(8), 613–24.

[2] Keseler, I. M., Bonavides-Martinez, C., Collado-Vides, J., Gama-Castro, S., Gunsalus, R. P., Johnson, D. A., Krummenacker, M., Nolan, L. M., Paley, S., Paulsen, I. T., Peralta-Gil, M., Santos-Zavaleta, A., Shearer, A. G., & Karp, P. D. 2009. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res, 37(Database issue), D464–70. [3] Goldenbaum, P. E., & Hall, G. A. 1979. Transport of cyclic adenosine 3’,5’-monophosphate across Escherichia coli vesicle membranes. J Bacteriol, 140(2), 459–67.

[4] Makman, R. S., & Sutherland, E. W. 1965. Adenosine 3’,5’-Phosphate in Escherichia Coli. J Biol Chem, 240, 1309–14.

[5] K Mori and H Aiba

[6] Cell. 1983 Jan;32(1):141-9. Autoregulation of the Escherichia coli crp gene: CRP is a transcriptional repressor for its own gene. Aiba H

[7] Rollie S. Ackerman, Nicholas R. Cozzarelli and Wolfgang Epstein.Accumulation of Toxic Concentrations of Methylglyoxal by Wild-Type Escherichia coli K-12.J. Bacteriol. August 1974 vol. 119 no. 2 357-362

[8] Jan Weber†, Anke Kayser‡ and Ursula Rinas. Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. II. Dynamic response to famine and feast, activation of the methylglyoxal pathway and oscillatory behaviour. Microbiology March 2005 vol. 151 no. 3 707-716