Coding

Part:BBa_K808014

Designed by: Sascha Hein, Sven Rumpf, Daniel Sachs, Arne Wehling, Philipp Rottmann   Group: iGEM12_TU_Darmstadt   (2012-09-02)
Revision as of 21:27, 25 September 2012 by DSachs (Talk | contribs)

AroY: Catalyzes protocatechuate to catechol

Figure 1. Gaussian network modelling of AroY. The supposed membrane anchor in red. For simulation parameters http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY click here.

AroY codes for the protocatechuate deacarboxylase from Klebsiella pneumonie ssp. pneumonie. It has a molecular mass of 57 kDa and is reported to form a complex of five identical AroY subunits. [http://2012.igem.org/Team:TU_Darmstadt/Modeling_GNM#AroY Gaussian network modelling] showed a flexible helix linker. This could be either a membrane anchor or responsible for the formation of a pentamer.


Usage and Biology

AroY is reported to catalyze the reaction of protocatechuate (3,4-Dihydroxybenzoate) to catechol under anaerobic conditions.







Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 37


[edit]
Categories
Parameters
protein