Part:BBa_K917005:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K917005

Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection.

Nitroreductase containing cells's growth (right) is markedly inhibited in comparison to control cells (left) at 90 ug/ml metronidazole. However, this inhibition is not absolute so cannot be used for counterselection.

Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing.

Liquid aerobic cultures of BS-nitroreductase and BS-control in the presence and absence of metronidazole. At 150 ug/ml metronidazole, cells which contain nitroreductase show almost no growth.The measurements were made in triplicate. Error bars are standard error of the mean. Inital OD600 (just after inoculation) was subtracted.

User Reviews

UNIQ2e95cc6665415f4f-partinfo-00000000-QINU UNIQ2e95cc6665415f4f-partinfo-00000001-QINU