Composite

Part:BBa_K733018:Design

Designed by: WANG, Yuqi   Group: iGEM12_HKUST-Hong_Kong   (2012-09-16)
Revision as of 17:02, 26 September 2012 by Yliac (Talk | contribs) (→‎Source)

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xylR+PxylA+RBS+GFP+Double Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2058


Design Notes

None.

Source

We digest and ligate our xylR+PxylA and BBa_E0240 from 2012 Kit Plate.

Backbone: xylR+PxylA in pSB1C3. Enzymes used: SpeI and PstI-HF.

Insert: BBa_E0240. Enzymes used: XbaI and PstI-HF. Purified by gel puficiation.

References