Part:BBa_K785000

Lov-tetR (light sensor with Ptet repression) light->PtetO repressed

This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light. This protein, which is actually a well designed fusion protein, include two domain. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline resistance, which could combine the tetracycline and enable the expression of the tetA. We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 106
  Illegal PstI site found at 160
  Illegal PstI site found at 289
  Illegal PstI site found at 626
  Illegal PstI site found at 940
  Illegal PstI site found at 1033
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 106
  Illegal PstI site found at 160
  Illegal PstI site found at 289
  Illegal PstI site found at 626
  Illegal PstI site found at 940
  Illegal PstI site found at 1033
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 106
  Illegal PstI site found at 160
  Illegal PstI site found at 289
  Illegal PstI site found at 626
  Illegal PstI site found at 940
  Illegal PstI site found at 1033
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 106
  Illegal PstI site found at 160
  Illegal PstI site found at 289
  Illegal PstI site found at 626
  Illegal PstI site found at 940
  Illegal PstI site found at 1033
  Illegal AgeI site found at 478
  
• 1000
  COMPATIBLE WITH RFC[1000]