Coding

Part:BBa_K896000

Designed by: Hsun-I Chiu   Group: iGEM12_NYMU-Taipei   (2012-09-09)
Revision as of 15:56, 16 September 2012 by Cutelucypoop (Talk | contribs)

SQR(sulfide quinone reductase),from Synechococcus sp. PCC 7002 plasmid pAQ7 

 SQR introduction:

Sulfide-dependent anoxygenic photosynthesis, driven by photosystem I (PS/I) alone, among cyanobacteria was first described for Oscillatoria limnetica from Solar Lake. Later photosynthetic sulfide oxidation in O. limnetica led to the discovery of sulfide-quinone reductase (SQR; E.C.1.8.5.′), a novel enzyme that transfers electrons from sulfide into the quinone pool. 

 Cloning of SQR gene:

SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene. File:SQR cloning.tif


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 349


[edit]
Categories
Parameters
None