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Part:BBa_K553010:Experience

Designed by: Veronica Parisi   Group: iGEM11_UNITS_Trieste   (2011-10-25)
Revision as of 10:48, 1 November 2011 by Giulio (Talk | contribs) (→‎Applications of BBa_K553010)

Datasheet download - experience inside: Media:K553010_lasIcost.pdf‎

Applications of BBa_K553010

UNITS_Trieste Synbiome project

bacteria Intra-strain communication: An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule (strain A). The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria (strain B).

The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8.

Coculture assay.jpg

The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes.

HPLC-MS/MS assay of an organic extract of strain A with BBa_K553010 in a medium copy number plasmid.

Ms oc 12.jpg

Blue: culture media 20x concentrated + OC8 and OC12 AHL Standard 1uM - 10 uL loaded Red: strain A organic extract 20x concentrated - 10 uL loaded

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