Part:BBa_K633001
Linker + EstA Membrane Protein EstA is an outer membrane-anchored esterase from Pseudomonas aeruginosa. An inactive EstA variant was used as an anchoring motif for the Escherichia coli cell-surface display of lipolytic enzymes.
EstA is an autotransporter protein which consists of an N-terminal domain harboring the catalytic activity and a C-terminal domain forming a b-barrel-like structure inserted into the bacterial outer membrane which mediates the translocation of the N-terminal domain.
The successful cell-surface display of lipases as fusion proteins to an inactive variant of EstA has already been described in several papers, where the passenger enzymes retain their hydrolytic activities after being anchored on the outer surface of E. coli cells. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 217
- 1000COMPATIBLE WITH RFC[1000]
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