Coding

Part:BBa_K559001:Design

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM11_Hong_Kong-CUHK   (2011-09-29)
Revision as of 22:24, 14 October 2011 by Jacky Loo (Talk | contribs) (→‎Source)

Halorhodopsin under T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 368
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 380
    Illegal AgeI site found at 106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 316


Design Notes

The coding sequence of N.pharaonis J05199.1 is selected from 61nt to 700nt and the T7 promotor with lac operon and RBS placed in front of the BBa_K559000 to get constitutive expression of its membrane protein.

Source

Halorhodopsin come from N.pharaonis, gene bank J05199.1

T7 promoter with lac operon was extracted from pET-23b sequence 303-348 nt and RBS was added from the primers for PCR amplification.

The above gene is assembled with our halorhodopsin gene BBa_K559000 by 3A assembly, leaving a scar of 'tactag'

References