Part:BBa_K518004:Experience
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Applications of BBa_K518004
User Reviews
UNIQ0e7d40444d1cfa69-partinfo-00000000-QINU
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UT-Tokyo 2011 |
We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but failed to do that. In the previous works (Chao, Lo, & Luo, 2000), the amount of L-Asp was determined by HPLC. We, however, were unable to use this machinery, so we tried to detect it through alternative method, ninhydrin reaction and ultraviolet-visible spectroscopy. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo our result page]. Reference Chao, Y., Lo, T., & Luo, N. (2000). Selective production of L-aspartic acid and L-phenylalanine by coupling reactions of aspartase and aminotransferase in Escherichia coli. Enzyme Microb Technol, 27(1-2), 19-25.
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