Generator

Part:BBa_K518004:Experience

Designed by: Masato Ohgishi   Group: iGEM11_UT-Tokyo   (2011-09-15)
Revision as of 17:43, 3 October 2011 by Mogi (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K518004

User Reviews

UNIQ704474999136d029-partinfo-00000000-QINU

UT-Tokyo 2011

We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but could not. Our sequencing results indicated a general sequence agreement, showing only a few mismatching bases. We have tried to detect the L-Asp by either ninhydrin staining or ultraviolet-visible spectroscopy, only to fail. Ninhydrin reactedwith not only amino acid (Asp) but indeed ammonium ion itself, and U-V spectroscopy detected large fumarate noise. We also tried to directly measure intracellular AspA over-expression, but have little days to this work.

UNIQ704474999136d029-partinfo-00000002-QINU