RNA

Part:BBa_K598006:Design

Designed by: Siyang HAO   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 06:25, 3 October 2011 by Hsy (Talk | contribs) (→‎Source)

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Theophylline Responsive Riboswitch 1G1 with Engineered RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed to up-regulate downstream gene expression in the presence of theophylline. In order to obtain this part for further utilization,get it by PCR, rather than enzyme digestion.

Source

Based on original design of Beatrix Suess, we change the RBS to a more robust one. Insert RNA controller sequence into backbone pSB1C3 via PCR and bluntend ligation.

References

Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.