Reporter
rLuc

Part:BBa_K518001

Designed by: Masato Ohgishi   Group: iGEM11_UT-Tokyo   (2011-09-15)
Revision as of 15:14, 29 September 2011 by Mogi (Talk | contribs)

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RBS + renilla luciferase + d.terminator

Luciferase from sea pansy (Renilla reniformis). Renilla luciferase emits light by the oxidation of coelenterazine, its substrate. Luciferase can be used as a measuring tool when combined with other cis-elements (regions regulating the expression of genes on the same DNA molecule; promoters and other protein-binding domains), because of its highly quantitative performance.

Molecular Biology

Renilla luciferase, a monomeric 36,000 Dalton protein, catalyzes coelenterazine oxidation to emit luminescence of around 480nm.

Usage

For detailed data, see [http://2011.igem.org/Team:UT-Tokyo our page] and BBa_K518002.

Reference

Matthews, J.C., et al., “Purification and properties of Renilla reniformis luciferase.”, Biochemistry, 16, 85 (1977)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 566


[edit]
Categories
Parameters
emission480nm