Composite

Part:BBa_K584019:Design

Designed by: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne   Group: iGEM11_KULeuven   (2011-09-16)
Revision as of 11:09, 18 September 2011 by Vinchhi (Talk | contribs) (→‎References)

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OmpA_transmembrane_domain + Linker + point_mutant_HPLC6(AFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A point mutation at Base Pair number 156 of the HPLC6 sequence was performed by replacing the T in the wild-type coding sequence to C.This mutation was necessary as the sequence CTGCAG in the Wild-type coding sequence corresponded to a Pst1 restriction site. GCT(Wild type) and GCC(1 point mutant) are both valid triplet codons for Alanine.


Source

SOURCE - Pseudopleuronectes americanus (winter flounder) Gene - HPLC6


References

http://www.ncbi.nlm.nih.gov/nuccore/M62417.1