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Coding
BisdAB

Part:BBa_K525517

Designed by: Jonas Aretz   Group: iGEM11_Bielefeld-Germany   (2011-09-14)
Revision as of 13:34, 17 September 2011 by Jaretz (Talk | contribs)

Fusion protein of BisdA and BisdB behind constitutive promoter

Fusion protein of BisdA and BisdB behind constitutive promoter.


Usage and Biology

Expressing this BioBrick in E. coli enables the bacterium to degrade the endocrine disruptor bisphenol A (BPA).


Important parameters

Experiment Characteristic Result
Expression in E. coli
Compatibility E. coli KRX, TOP10, MACH1, BL21(DE3)
Expression Constitutive
Optimal temperature 30 °C
Working BPA concentration <120 mg L-1
Purification Molecular weight 59.3 kDa
Theoretical pI 4.99
High absorbation 450 nm
Degradation of BPA Completely degradation of 0.53 mM BPA 21 - 24 h
Specific BPA degradation rate 1.29 10-10 mM cell-1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 664
    Illegal BamHI site found at 1402
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal AgeI site found at 1655
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/degradation/bisphenol
//proteindomain/internal
Parameters
n/a Fusion protein of BisdA and BisdB behind constitutive promoter