Regulatory
PalcA

Part:BBa_K678001:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-02)
Revision as of 07:52, 12 September 2011 by Jaide (Talk | contribs) (→‎Design Notes)

PalcA, inducible Aspergillus nidulans promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.

Promotor_med_haler.png

This part was USER cloned into the shipping plasmid pSB1C3 but is also contained in the Plug'n'Play plasmids pJEJAM10, pJEJAM11, pJEJAM19, and pJEJAM20

Source

Aspergillus nidulans genomic DNA

References

(1) Lubertozzi, D., and Keasling, J.D.: Marker and promoter effects on heterologous expression in Aspergillus nidulans. Appl Microbiol Biotechnol (2006) 72: 1014–1023.

(2) Creaser, E.H., Porter, R.L., Britt, K.A., Pateman, J.A. and, Doy, C.H.: Purification and preliminary characterization of alcohol dehydrogenase from Aspergillus nidulans. Biochem. J. (1985) 225: 449-454.

(3) Bailey, C., and Arst Jr., H.N.: Carbon catabolite repression in Aspergihs nidulans. Eur. J. Biochem. (1975) 51: 573-577.

(4) Waring, R.B., May, G.S., and Morris, N.R.: Characterization of an inducible expression system in Aspergillus nidulans using alcA and tuhulin- coding genes. Gene, (1989) 79: 119-130.

(5) Romero, B., Turner, G., Olivas, I., Laborda, F., and De Lucas, J.R.: The Aspergillus nidulans alcA promoter drives tightly regulated conditional gene expression in Aspergillus fumigatus permitting validation of essential genes in this human pathogen. Fungal Genetics and Biology (2003) 40: 103–114.