Translational_Unit

Part:BBa_K376004:Design

Designed by: Andrew Kirk   Group: iGEM10_Penn_State   (2010-10-26)
Revision as of 21:31, 6 November 2010 by Marxspiro (Talk | contribs) (→‎References)

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Anaerobic Fluorescent Protein with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein was codon optimized for E. coli by DNA 2.0 and the the RBS was optimized using the Salis Lab RBS calculator http://salis.psu.edu/RBS_Calculator.shtml

Source

Protein sequence was taken from:

Tielker D, Eichhof I, Jaeger KE, Ernst JF. Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae. Eukaryot Cell. 2009 (6):913-915

References

Tielker D, Eichhof I, Jaeger KE, Ernst JF. 2009. Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae. Eukaryot Cell. 2009 (6):913-915

Salis HM, Mirsky EA, Voigt CA. 2009. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol. 2009 Oct;27(10):946-50