Difference between revisions of "Part:BBa K371019"
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We use the ultracentrifuge to purify the proteins, with 48000 g speed, we get our targeted proteins in the pellet. | We use the ultracentrifuge to purify the proteins, with 48000 g speed, we get our targeted proteins in the pellet. | ||
| − | [[Image:Pdu_Sds-page.jpg]] | + | [[Image:Pdu_Sds-page.jpg]] [[Image:2010ustc-dj4.JPG]] |
Revision as of 02:54, 29 October 2010
pdu(Propanediol utilization)BMC shell gene under Plac control
This composite consists of eight pdu bacterial microcompartment(BMC) structure gene following a natural lac promotor(BBa_R0010). These genes come from four basic parts,pduAB,pduJK,pduN and pduTUV, which consist of the natural RBS and gene separately.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1894
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3236
- 1000COMPATIBLE WITH RFC[1000]
In the expression of protein, we use and unite the method mentioned in the previous papers. [see protocol] The bacterial was induced by 1 mM IPTG at 30 ℃ for 4 hours. The results was as follows.[the one without pduN was a negative control used to prove the function of pduN: in the growth process, the one without pduN tends to grow with more deposit implying its possible role in the vertex]
We use the ultracentrifuge to purify the proteins, with 48000 g speed, we get our targeted proteins in the pellet.
