Difference between revisions of "Part:BBa K415506"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K415506 short</partinfo> | <partinfo>BBa_K415506 short</partinfo> | ||
− | + | Modified TRE promoter with six tetO sites to provide low basal transcriptional activity, a significant improvement over the previously used simple TRE promoter. Upon induction with rtTA or rtTA variants, and the addition of doxycycline (DOX), the transcriptional activity of TREt drastically increases. | |
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+ | ==Characterization== | ||
+ | [[Image:TREt_characterization.jpg|thumb|left|Figure 1. DOX Induction of TREt Promoter Post Calcium Phosphate Transfection of HEK293 rtTA3 Cell Line.]] Response of K415506 to DOX induction. | ||
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+ | DOX addition occured with medium change post calcium phosphate transfection of HEK293 rtTA3 cell line with TREt_EGFP construct. | ||
− | + | '''Results''' | |
− | + | DOX addition led to dramatic increase in transcriptional activity, as seen in Figure 1. Basal transcription level is shown to be low in the left panel. | |
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+ | ==Sequence and Features== | ||
<partinfo>BBa_K415506 SequenceAndFeatures</partinfo> | <partinfo>BBa_K415506 SequenceAndFeatures</partinfo> | ||
Revision as of 01:32, 29 October 2010
pTRE-Tight L4R1 MammoBlock
Modified TRE promoter with six tetO sites to provide low basal transcriptional activity, a significant improvement over the previously used simple TRE promoter. Upon induction with rtTA or rtTA variants, and the addition of doxycycline (DOX), the transcriptional activity of TREt drastically increases.
Characterization
Response of K415506 to DOX induction.DOX addition occured with medium change post calcium phosphate transfection of HEK293 rtTA3 cell line with TREt_EGFP construct.
Results DOX addition led to dramatic increase in transcriptional activity, as seen in Figure 1. Basal transcription level is shown to be low in the left panel.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30 - 1000COMPATIBLE WITH RFC[1000]