Difference between revisions of "Part:BBa K371030"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K371030 short</partinfo> | <partinfo>BBa_K371030 short</partinfo> | ||
| Line 17: | Line 16: | ||
<partinfo>BBa_K371030 parameters</partinfo> | <partinfo>BBa_K371030 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| + | [[Image:USTC2010_Result_of_Fusion_Protein_P_L-GFP(1).]] | ||
Revision as of 01:17, 29 October 2010
MPF(meta-prefix)+[pduP64+10*GS+GFP] fusion protein+MSF(meta-suffix)
This parts is a fusion protein between the N terminus 64 amino acid of pdu P protein and GFP protein(BBa_E0043). At each end is special DNA sequence that make this parts compatible to BBF RFC 53. The MPF and MSF is the abbreviation of meta-prefix and meta-suffix seperately. Because the N terminus is said to target pduP inside the pdu BMC, this part can be used to direct pduP-GFP chimera into BMC lumun.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 882
Illegal SapI site found at 3