Difference between revisions of "Part:BBa K415513"
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<partinfo>BBa_K415513 short</partinfo>
 
<partinfo>BBa_K415513 short</partinfo>
  
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L4R1 MammoBlock Promoter Vector. Contains the Wnt-Responsive Element from the Lef1 promoter, in front of the SV40 minimal promoter. Candidate mechanosensitive promoter.  
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L4R1 MammoBlock Promoter Vector. Synthetic construct of:
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* The Wnt-Responsive Element from the Lef1 promoter
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* SV40 minimal constitutive promoter.  
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Wnt has been shown to be heavily involved in many transduction pathways induced by mechanical stress. The WRE motif is suspected of being able to offer mechanosensitivity to synthetic promoters, particularly to adhesion and strain.
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==References==
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Hughes-Fulford, M. "Signal Transduction and Mechanical Stress." Science STKE 2004.249 (2004). Print.
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Nelson, W. J. "Convergence of Wnt,  B-Catenin, and Cadherin Pathways." Science 303.5663 (2004): 1483-487. Print.
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K415513 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K415513 SequenceAndFeatures</partinfo>

Revision as of 00:19, 29 October 2010

pWRE_SV40 L4R1 MammoBlock Entry Vector

L4R1 MammoBlock Promoter Vector. Synthetic construct of:

  • The Wnt-Responsive Element from the Lef1 promoter
  • SV40 minimal constitutive promoter.

Wnt has been shown to be heavily involved in many transduction pathways induced by mechanical stress. The WRE motif is suspected of being able to offer mechanosensitivity to synthetic promoters, particularly to adhesion and strain.

References

Hughes-Fulford, M. "Signal Transduction and Mechanical Stress." Science STKE 2004.249 (2004). Print. Nelson, W. J. "Convergence of Wnt, B-Catenin, and Cadherin Pathways." Science 303.5663 (2004): 1483-487. Print.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal BglII site found at 155
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]