Difference between revisions of "Part:BBa K200029:Experience"
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Revision as of 21:40, 28 October 2010
Experience of UW-Madison 2010
The following is a zip file containing pertinent testing protocols and results, sequencing, and genbank files: media:BBa_K318501_pSB1C3.zip
Figure 1: L-Fucose content of plasmids K318500, K318501(K200029), and K200021 (control) in backbone pSB1AK3 transformed in MG1655. Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Colonic acid can be estimated by measuring L-fucose content.
Figure 2: Survivability of plasmids K318500, K318501(K200029), K318502, and K200021 (control) in backbone pSB1AK3 transformed in MG1655. We found different results compared to the survivability testing preformed by Imperial 2009. We saw around a 10x increase in survivability with RcsB being over produced at a pH=4.
For more detail on testing, please see our results page on our Team Wiki for protocols, background information, and conclusions.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Applications of BBa_K200029
User Reviews
UNIQ22367390c44e147a-partinfo-00000002-QINU UNIQ22367390c44e147a-partinfo-00000003-QINU