Difference between revisions of "Part:BBa K415503"

Revision as of 20:55, 28 October 2010

EYFP_2a_rtta3_ff5 L1L2 MammoBlock Entry Vector

EYFP_2a_rtTa3_FF5 is a construct containing two proteins - EYFP fluorescent protein, and rtTA3 (a DOX-inducible transcription factor that regulates the TRE promoter) seperated by a viral 2A sequence that allows for bicistronic expression of the two genes. FF5 is a micro-RNA controlled degradation tag. This construct is part of the 2010 iGEM mammalian team toggle switch.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1666
Illegal XbaI site found at 816
Illegal XbaI site found at 1709
Illegal PstI site found at 225
Illegal PstI site found at 1671
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1666
Illegal NheI site found at 804
Illegal PstI site found at 225
Illegal PstI site found at 1671
Illegal NotI site found at 1560
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1666
Illegal BamHI site found at 1697
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1666
Illegal XbaI site found at 816
Illegal XbaI site found at 1709
Illegal PstI site found at 225
Illegal PstI site found at 1671
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1666
Illegal XbaI site found at 816
Illegal XbaI site found at 1709
Illegal PstI site found at 225
Illegal PstI site found at 1671
Illegal NgoMIV site found at 774
Illegal NgoMIV site found at 1433
Illegal AgeI site found at 8
1000
COMPATIBLE WITH RFC[1000]

Revision as of 17:52, 28 October 2010 (view source) Ldeming (Talk \| contribs) ← Older edit		Revision as of 20:55, 28 October 2010 (view source) Ldeming (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K415503 short</partinfo>		<partinfo>BBa_K415503 short</partinfo>

−	~~EYFP_2a_rtTa3_ff5~~ is a construct containing two proteins - EYFP fluorescent protein, and rtTA3 (a DOX-inducible transcription factor) seperated by a viral 2A sequence that allows for bicistronic expression of the two genes. FF5 is a micro-RNA controlled degradation tag. This construct as part of the 2010 iGEM mammalian team toggle switch.	+	EYFP_2a_rtTa3_FF5 is a construct containing two proteins - EYFP fluorescent protein, and rtTA3 (a DOX-inducible transcription factor that regulates the TRE promoter) seperated by a viral 2A sequence that allows for bicistronic expression of the two genes. FF5 is a micro-RNA controlled degradation tag. This construct is part of the 2010 iGEM mammalian team toggle switch.