Difference between revisions of "Part:BBa K415505"

 
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<partinfo>BBa_K415505 short</partinfo>
 
<partinfo>BBa_K415505 short</partinfo>
  
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MIT iGEM 2010 Mammalian Part
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Mammalian L1L2 Entry Vector containing VgEcR_2a_rxr_2a_hygro construct. VgEcR and RxR form a heterodimer to control expression of the EGSH promoter; they can be activated by the addition of the PonS compound. Here they are separated by a viral 2A site that allows for bicistronic expression. Hygromycin is a selection marker that protects mammalian cells against the addition of the Hygromycin antibiotic.
  
 
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Revision as of 18:16, 28 October 2010

VgEcR_2A_RxR L1L2 MammoBlock Entry Vector

Mammalian L1L2 Entry Vector containing VgEcR_2a_rxr_2a_hygro construct. VgEcR and RxR form a heterodimer to control expression of the EGSH promoter; they can be activated by the addition of the PonS compound. Here they are separated by a viral 2A site that allows for bicistronic expression. Hygromycin is a selection marker that protects mammalian cells against the addition of the Hygromycin antibiotic.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2091
    Illegal PstI site found at 2272
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1585
    Illegal BamHI site found at 486
    Illegal BamHI site found at 2962
    Illegal BamHI site found at 3115
    Illegal BamHI site found at 3229
    Illegal XhoI site found at 1578
    Illegal XhoI site found at 1659
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 291
    Illegal PstI site found at 2272
    Illegal NgoMIV site found at 1707
    Illegal NgoMIV site found at 2054
    Illegal NgoMIV site found at 3527
    Illegal AgeI site found at 2815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3000
    Illegal SapI.rc site found at 389
    Illegal SapI.rc site found at 521