Difference between revisions of "Part:BBa K352011"
| Line 6: | Line 6: | ||
<br> PcooF and PcooM, the two CooA-regulated R. rubrum promoters, contain 2-fold symmetric DNA sequences which are CooA-binding sites and are similar to the CRP consensus sequence. This similarity corralates with the tantamount helix-turn-helix motifs CooA and CRP. The CooA binding sites lie at the 243.5 and 238.5 positions relative to the transcription start sites in PcooF and PcooM, respectively, overlapping with the 235 region. This overlap suggests that both CooA-regulated promoters are analogous to class II CRP-dependent promoters. We chose PcooF for this study because it is the stronger promoter based on the amount of primer extension product and level of coo-encoded proteins synthesized in vivo. | <br> PcooF and PcooM, the two CooA-regulated R. rubrum promoters, contain 2-fold symmetric DNA sequences which are CooA-binding sites and are similar to the CRP consensus sequence. This similarity corralates with the tantamount helix-turn-helix motifs CooA and CRP. The CooA binding sites lie at the 243.5 and 238.5 positions relative to the transcription start sites in PcooF and PcooM, respectively, overlapping with the 235 region. This overlap suggests that both CooA-regulated promoters are analogous to class II CRP-dependent promoters. We chose PcooF for this study because it is the stronger promoter based on the amount of primer extension product and level of coo-encoded proteins synthesized in vivo. | ||
| + | == Imaging with confocal laser scanning microscopy == | ||
| + | <html><div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/a/ae/Confocal_sol.JPG"></a></div></html> | ||
<!-- --> | <!-- --> | ||
Revision as of 16:56, 28 October 2010
pCooM Promoter coupled with RBS+RFP+Double terminator
pCooM Promoter coupled with RBS, RFP,double terminator
PcooF and PcooM, the two CooA-regulated R. rubrum promoters, contain 2-fold symmetric DNA sequences which are CooA-binding sites and are similar to the CRP consensus sequence. This similarity corralates with the tantamount helix-turn-helix motifs CooA and CRP. The CooA binding sites lie at the 243.5 and 238.5 positions relative to the transcription start sites in PcooF and PcooM, respectively, overlapping with the 235 region. This overlap suggests that both CooA-regulated promoters are analogous to class II CRP-dependent promoters. We chose PcooF for this study because it is the stronger promoter based on the amount of primer extension product and level of coo-encoded proteins synthesized in vivo.
Imaging with confocal laser scanning microscopy
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 647
Illegal AgeI site found at 759 - 1000COMPATIBLE WITH RFC[1000]