Difference between revisions of "Part:BBa K352017"
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<br> This component is used to measure high levels of carbon monoxide in the environment. Construction of this device was performed by utilizing strong CO sensitive promoter which is pCooM promoter from Rhodospirillum rubrum. This device is only active when there is CooA transcriptional activator and Carbon monoxide gas in the environment. When there is CO in the environment, the device produces red fluorescent protein in response.
 
<br> This component is used to measure high levels of carbon monoxide in the environment. Construction of this device was performed by utilizing strong CO sensitive promoter which is pCooM promoter from Rhodospirillum rubrum. This device is only active when there is CooA transcriptional activator and Carbon monoxide gas in the environment. When there is CO in the environment, the device produces red fluorescent protein in response.
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Agar plates:
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Our Agar plates with pCooM-RBS-RFP-TT-pLac-RBS-CooA showed that because of low level of constitutive binding of CooA to pCooM promoter, we have observed low level of RFP fluorescence on agar plates.
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<html><div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 350px; height: 250px;" alt="w7" src="https://static.igem.org/mediawiki/2010/f/ff/Agar_plate_sol.JPG"></a></div> </html>
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<html><div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 350px; height: 250px;" alt="w7" src="https://static.igem.org/mediawiki/2010/6/67/Agar_Plate_sa%C4%9F.JPG"></a></div> </html>
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==Culturing CO Sensor E.coli with CO induction==
 
==Culturing CO Sensor E.coli with CO induction==

Revision as of 16:38, 28 October 2010

pCooM RBS RFP Double terminator CooA coupled with placI+RBS

This part is formed by combination of pCooM Promoter coupled with RBS+RFP+Double terminator and CooA coupled with placI+RBS.


This component is used to measure high levels of carbon monoxide in the environment. Construction of this device was performed by utilizing strong CO sensitive promoter which is pCooM promoter from Rhodospirillum rubrum. This device is only active when there is CooA transcriptional activator and Carbon monoxide gas in the environment. When there is CO in the environment, the device produces red fluorescent protein in response.


Culturing CO Sensor E.coli with CO induction

Usage and Biology

w7


We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
    Illegal AgeI site found at 759
    Illegal AgeI site found at 1617
  • 1000
    COMPATIBLE WITH RFC[1000]