Difference between revisions of "Part:BBa K352017:Experience"

Revision as of 16:36, 28 October 2010

Signal Quantification of pCooM-RBS-RFP-TT-pLac-RBS-CooA:

We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.

The data shows that RFP fluorescence increases with CO induction.

Imaging with confocal laser scanning microscopy

Applications of BBa_K352017

User Reviews

UNIQ30c5449934d996fb-partinfo-00000003-QINU UNIQ30c5449934d996fb-partinfo-00000004-QINU

@@ Line 14: / Line 14: @@
 <html><div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/a/ae/Confocal_sol.JPG"></a></div></html>
 <html><div  align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/2/26/Confocal_sa%C4%9F.JPG"></a></div> </html>
-==Culturing CO Sensor E.coli with CO induction==
-<html><div  align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 500px; height: 200px;" alt="w7" src="https://static.igem.org/mediawiki/2010/4/49/Culturing_alpha_1.JPG"></a></div> </html>
-<br> We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.