Difference between revisions of "Part:BBa K352011:Design"
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<br> The sequence information was acquired from NCBI and physical DNA of pcooM was synthesized from GENEART. Standart Biobrick assembly strategies(restriction digestion) were used to produce biobricks. | <br> The sequence information was acquired from NCBI and physical DNA of pcooM was synthesized from GENEART. Standart Biobrick assembly strategies(restriction digestion) were used to produce biobricks. | ||
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===References=== | ===References=== | ||
Latest revision as of 15:51, 28 October 2010
pCooM Promoter coupled with RBS+RFP+Double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 647
Illegal AgeI site found at 759 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Device created from a composite of parts.
The sequence information was acquired from NCBI and physical DNA of pcooM was synthesized from GENEART. Standart Biobrick assembly strategies(restriction digestion) were used to produce biobricks.
References
- Aono S, Ohkubo K, Matsuo T, and Nakajima T (1998). Redox-controlled ligand exchange of the Heme in the CO-sensing transcriptional activator CooA. The Journal of Biologycal Chemistry 1998; 273(40): 25757-64.
- Aono S (2003). Biochemical and Biophysical Properties of the CO-Sensing Transcriptional Activator CooA. Accounts of Chemical Research 2003; 36(11): 825-831.