Difference between revisions of "Part:BBa K352011:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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== Signal Quantification of pCooM-RBS-RFP-TT-pLac-RBS-CooA: ==
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<br> We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.
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<html><div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 900px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/b/b6/BU_SEFER_TAMAM.JPG"></a></div> </html>
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<br> The data shows that RFP fluorescence increases with CO induction.  
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== Imaging with confocal laser scanning microscopy ==
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<html><div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/a/ae/Confocal_sol.JPG"></a></div></html>
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<html><div  align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/2/26/Confocal_sa%C4%9F.JPG"></a></div> </html>
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==Culturing CO Sensor E.coli with CO induction==
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<html><div  align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 500px; height: 200px;" alt="w7" src="https://static.igem.org/mediawiki/2010/4/49/Culturing_alpha_1.JPG"></a></div> </html>
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<br> We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.
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===Applications of BBa_K352011===
 
===Applications of BBa_K352011===

Revision as of 14:56, 28 October 2010


Signal Quantification of pCooM-RBS-RFP-TT-pLac-RBS-CooA:


We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.


w7

The data shows that RFP fluorescence increases with CO induction.



Imaging with confocal laser scanning microscopy

w7
w7


Culturing CO Sensor E.coli with CO induction

w7


We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.


Applications of BBa_K352011

User Reviews

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