Difference between revisions of "Part:BBa K352017:Experience"

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<br> We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.
  
  

Revision as of 13:58, 28 October 2010



Signal Quantification of pCooM-RBS-RFP-TT-pLac-RBS-CooA:


We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.


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The data shows that RFP fluorescence increases with CO induction.


Imaging with confocal laser scanning microscopy

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Culturing CO Sensor E.coli with CO induction

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We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.


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