Difference between revisions of "Part:BBa K352017"
| Line 8: | Line 8: | ||
| − | <html><div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: | + | <html><div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 350px; height: 250px;" alt="w7" src="https://static.igem.org/mediawiki/2010/f/ff/Agar_plate_sol.JPG"></a></div> </html> |
| − | <html><div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: | + | <html><div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 350px; height: 250px;" alt="w7" src="https://static.igem.org/mediawiki/2010/6/67/Agar_Plate_sa%C4%9F.JPG"></a></div> </html> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 13:54, 28 October 2010
pCooM RBS RFP Double terminator CooA coupled with placI+RBS
This part is formed by combination of pCooM Promoter coupled with RBS+RFP+Double terminator and CooA coupled with placI+RBS.
Agar plates: Our Agar plates with pCooM-RBS-RFP-TT-pLac-RBS-CooA showed that because of low level of constitutive binding of CooA to pCooM promoter, we have observed low level of RFP fluorescence on agar plates.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 647
Illegal AgeI site found at 759
Illegal AgeI site found at 1617 - 1000COMPATIBLE WITH RFC[1000]