Difference between revisions of "Part:BBa K305011:Design"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K305011 short</partinfo> | <partinfo>BBa_K305011 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | |||
| + | Contains a BamHI restriction site in between the prefix and suffix. | ||
Revision as of 04:00, 28 October 2010
Subtilin inducible expression plasmid for Bacillus subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3141
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3147 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3141
Illegal BglII site found at 3060
Illegal XhoI site found at 287 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3141
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3141
Plasmid lacks a suffix.
Illegal XbaI site found at 3156
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI site found at 324
Design Notes
Contains a BamHI restriction site in between the prefix and suffix.
Source
...