Difference between revisions of "Part:BBa K399000:Design"

(→‎Design Notes)
 
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
The coding sequence was on a pET28a plasmid bought from Invitrogen by the DeLisa lab at Cornell University.
+
mHoneydew was developed by Roger Tsien<sup>1</sup>.  
  
 
===References===
 
===References===
 +
1.  Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572

Latest revision as of 03:34, 28 October 2010

mHoneydew


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 340
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mHoneydew contains a PstI site, so do not digest it with PstI. To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and Xba1. Digest the upstream sequence at EcoRI and Spe1.

Source

mHoneydew was developed by Roger Tsien1.

References

1. Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572