Difference between revisions of "Part:BBa K319005:Design"

 
(→‎Source)
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K319005 short</partinfo>
 
<partinfo>BBa_K319005 short</partinfo>
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
The part was extracted from S. cerevisiae, YPH500 strain, using colony PCR protocols. It consists of 720 bp upstream of ADH1 gene according to SGD sequence.  
+
The part was extracted from ''S. cerevisiae'' YPH500 strain using colony PCR protocols. It consists of 720 bp upstream of ADH1 gene. The primers were designed according to the genomic sequence found on [http://www.yeastgenome.org ''Saccharomyces'' Genome Database]. The design, extraction, and cloning the BioBrick part were performed by Afnan Azizi of 2010 uOttawa iGEM team.
  
 
===References===
 
===References===

Latest revision as of 03:33, 28 October 2010

yeast mid-length ADH1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93


Design Notes

There were three options for the length of the promoter - short, middle, and long. The middle length was chosen.


Source

The part was extracted from S. cerevisiae YPH500 strain using colony PCR protocols. It consists of 720 bp upstream of ADH1 gene. The primers were designed according to the genomic sequence found on [http://www.yeastgenome.org Saccharomyces Genome Database]. The design, extraction, and cloning the BioBrick part were performed by Afnan Azizi of 2010 uOttawa iGEM team.

References