Difference between revisions of "Part:BBa K313016"
 
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lox71 and lox66 are arranged so that the intermediate Tet resistance(reverse)-rbs(reverse) is inverted when Cre is expressed.
 
lox71 and lox66 are arranged so that the intermediate Tet resistance(reverse)-rbs(reverse) is inverted when Cre is expressed.
 
So, this unit can be used as a detector of Cre, because cells with this construct will survive in the medium with tetracycline only when the cells express Cre.
 
So, this unit can be used as a detector of Cre, because cells with this construct will survive in the medium with tetracycline only when the cells express Cre.
 +
 +
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:27, 28 October 2010

cre recombinase assay device

This part is composed of "T7 promoter-lox71-Tet resistance(reverse)-rbs(reverse)-lox66-double terminator."

lox71 and lox66 are arranged so that the intermediate Tet resistance(reverse)-rbs(reverse) is inverted when Cre is expressed. So, this unit can be used as a detector of Cre, because cells with this construct will survive in the medium with tetracycline only when the cells express Cre.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1139
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 993
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 599
    Illegal NgoMIV site found at 967
  • 1000
    COMPATIBLE WITH RFC[1000]