Difference between revisions of "Part:BBa K325202"

 
 
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LRE (Luciferin Regenerating Enzyme) is a protein found in the light producing lantern organs of fireflies. It catalyses the conversion of oxyluciferin back into luciferin upon the addition of D-cysteine, relieving inhibition of luciferase by oxyluciferin as well as fueling the luciferin pool of the cell.
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The ability of a cell to express LRE will increase both the brightness and glowing time of luciferin-requiring luciferases.
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For more information refer to our [http://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration wiki].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:46, 28 October 2010

Luciferin Regenerating Enzyme (LRE)
L. Cruciata
(E. coli optimised)


LRE (Luciferin Regenerating Enzyme) is a protein found in the light producing lantern organs of fireflies. It catalyses the conversion of oxyluciferin back into luciferin upon the addition of D-cysteine, relieving inhibition of luciferase by oxyluciferin as well as fueling the luciferin pool of the cell.

The ability of a cell to express LRE will increase both the brightness and glowing time of luciferin-requiring luciferases.

For more information refer to our [http://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 900
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 332