Difference between revisions of "Part:BBa K318517:Design"

Latest revision as of 02:04, 28 October 2010

Pc + RamA

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 159
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 159
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 159
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 159
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The ramA gene was PCR amplified from Salmonella enterica strain LT2 and was cloned behind the constitutive promoter J23100 to have RamA present in the cell. In our experiment, we were using RamA to bind to cholic acid (bile salt).

Source

The Salmonella enterica strain LT2 chromosomal DNA came from Diana Downs' Lab at the University of Wisconsin-Madison.

References

Nikaido E., A. Yamaguchi, and K. Nishino. 2008. AcrAB Multidrug Efflux Pump Regulation in Salmonella enterica serovar Typhimurium by RamA in Response to Environmental Signals. J. Biol. Chem. 283:24245-24253.

Revision as of 01:28, 28 October 2010 (view source) Msagstetter (Talk \| contribs) (→‎Design Notes) ← Older edit		Latest revision as of 02:04, 28 October 2010 (view source) Msagstetter (Talk \| contribs) (→‎References)
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	===References===		===References===
		+
		+	Nikaido E., A. Yamaguchi, and K. Nishino. 2008. AcrAB Multidrug Efflux Pump Regulation in ''Salmonella enterica'' serovar Typhimurium by RamA in Response to Environmental Signals. J. Biol. Chem. 283:24245-24253.