Difference between revisions of "Part:BBa K091107:Experience"
(→User Reviews) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 19: | Line 18: | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K091107 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K091107 EndReviews</partinfo> | ||
| + | |||
| + | Andrew Kirk, undergraduate, Penn State iGEM 2010 | ||
| + | |||
| + | Two Lux-inducible promoters that were already on the registry (K091107 and K091146) were characterized. Because these promoters require AHL and LuxR in order to activate, a construct was created that consisted of a Lux promoter followed by RFP, and a constitutive promoter followed by the LuxR gene. LuxR was expected to be present in excess so that the limiting factor would be AHL. The coding sequences for LuxR and RFP were preceded by the standard RBS B0034. | ||
| + | |||
| + | The AHL used was OC6-homoserine lactone. It was added to samples in a 96-well plate in concentrations of 0, .1, 1, 10, 100 and 1000nM. | ||
| + | |||
| + | The results [http://2010.igem.org/Team:Penn_State/Project#Lux_Promoter_Characterization on our wiki]show no trend in protein expression based on concentration of OC6-homoserine lactone. More research is warranted to provide information about what are the best conditions for chemically inducing the Lux promoters. | ||
Revision as of 01:39, 28 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K091107
User Reviews
UNIQ4aa42fca0c038746-partinfo-00000000-QINU UNIQ4aa42fca0c038746-partinfo-00000001-QINU
Andrew Kirk, undergraduate, Penn State iGEM 2010
Two Lux-inducible promoters that were already on the registry (K091107 and K091146) were characterized. Because these promoters require AHL and LuxR in order to activate, a construct was created that consisted of a Lux promoter followed by RFP, and a constitutive promoter followed by the LuxR gene. LuxR was expected to be present in excess so that the limiting factor would be AHL. The coding sequences for LuxR and RFP were preceded by the standard RBS B0034.
The AHL used was OC6-homoserine lactone. It was added to samples in a 96-well plate in concentrations of 0, .1, 1, 10, 100 and 1000nM.
The results [http://2010.igem.org/Team:Penn_State/Project#Lux_Promoter_Characterization on our wiki]show no trend in protein expression based on concentration of OC6-homoserine lactone. More research is warranted to provide information about what are the best conditions for chemically inducing the Lux promoters.